Figure 5. Effect of changes in pipette potential on single-channel current amplitude in cell-attached patches. (A) Representative tracings of single-channel activity taken at various pipette potentials (PP) in patches from two different cerebral arterial muscle cells, c - closed state of the channel. (B) The current-voltage (I-V) relationship for this channel obtained from six arterial membrane patches. The slope of the curve determined via linear regression analysis was 99 pS. Each point represents the mean plus/minus SEM.