Fig. 3.
Neuropathic injury provokes spinal phosphorylated histone deacetylase (pHDAC4) and 14-3-3β expressions and HDAC4-14-3-3β coupling. (A and B) Representative Western blots and statistical analysis showing, when compared with the sham operation (Sham), spinal nerve ligation (SNL) statistically up-regulated the level of pHDAC4 and total 14-3-3β (t14-3-3β) at days 3, 7, 14, and 21 after operation in the ipsilateral (I, IPSI) but not the contralateral (C, CONTRA) dorsal horn (**P < 0.01 compared with the Sham IPSI; ##P < 0.01 compared with day 1, n = 6). (C) Coimmunoprecipitation analysis showing SNL enhanced the tHDAC4-bound pHDAC4 and t14-3-3β- and t14-3-3β-bound pHDAC4 in the dorsal horn samples at days 3 and 7 after operation. In both cases, no detectable immunoreactivity was labeled by antibodies in the control IgG-recognized precipitation. GADPH = glyceraldehyde 3-phosphate dehydrogenase; IB = immunoblotting; IgG = immunoglobulin G; In = input control; IP = immunoprecipitation.