Steady-state binding study in phrenic nerve hemidiaphragm preparations in a tissue bath (n = 85). Mean and 95% CIs are provided. The EC₅₀ (the median effective concentration) of calabadion 2 and sugammadex in reversing rocuronium was within the same range (assessed by TOF, 61 vs. 49 μM; assessed by T1, 53 vs. 43 μM, respectively). (A) Concentration effect of rocuronium and cisatracurium on T1 depression. (B) Reversal of rocuronium- and cisatracurium-induced neuromuscular blockade by calabadion 2. (C) Reversal of rocuronium-induced neuromuscular blockade by sugammadex. TOF = train-of-four.