Fig. 8.
JM25-1 induced apoptosis of isolated human peripheral eosinophils. Eosinophils were cultured in the presence of interleukin-5 (30 ng/ml) for 72 h in the absence or presence of lidocaine or JM25-1. Column scatter graphs represent the percentage of cells in apoptosis. Eosinophils were treated with JM25-1 at 0.1 to 1 mM (A) or lidocaine (B). Horizontal lines represent the mean value and the open circles represent the median (n = 4, four different eosinophil donors). Results were analyzed by Kruskal–Wallis test followed by the Dunn multiple comparison test. *P < 0.05, **P < 0.01, and ***P < 0.001 versus untreated group.