Fig. 4.
Protein expression in primary human CD14+ cells and knockdown of nAchRα1 in human cell lines (A) and time course of cytokine response in SAEC after mechanical stretch for 48 h (B–I). CIS = cisatracurium; ECV304 = human endothelial cells; GM-CSF = granulocyte-macrophage colony-stimulating factor; IL-6 = interleukin-6; IL-8 = interleukin-8; nAchRα1 = nicotinic acetylcholine receptor α1; SAEC = primary human small airway epithelial cells; shAChRα1 = nAChRα1 small hairpin RNA; shCtrl = scrambled small hairpin RNA; siAChRα1 = nAChRα1 small-interfering RNA; siCtrl = scrambled small-interfering RNA; TNF-α = tumor necrosis factor-α. *P < 0.05 versus Stretch in (B–E). °P < 0.05 scrambled siRNA versus vehicle control; *P < 0.05 nAchαR1 siRNA versus scrambled siRNA and control in (F–I).