Figure 2. Locomotor activity in mice after isoflurane was stopped. Animals were placed in individual anesthetic chambers and given either 0%(control), 0.7%, or 1.5% isoflurane in 25% oxygen and 75% nitrogen (fresh gas flow, 4 l/min) for 20 min. Thereafter, isoflurane was stopped and the animals were exposed to 25% oxygen-balance nitrogen for another 20 min. Locomotor activity counts during the first (open bars) or the second (batched bars) 10-min period after cessation of exposure were compared. Each bar represents the mean +/- SEM of five animals. **P < 0.01 compared with control group, one-way analysis of variance with the least-significant-difference test.