AMG 517 does not prevent anesthesia-induced hypothermia in the absence of transient receptor potential vanilloid 1 (TRPV1) channels. (A) In wild-type (Trpv1^+/+) mice, ketamine (50 mg/kg intraperitoneally; i.p.) induced hypothermia that began within 20 min after the i.p. injection that sustained until the last measurement (240 min). However, when these animals were treated with AMG 517 (1 mg/kg i.p.), the ketamine-induced hypothermia was significantly prevented, starting at 40 min that persisted until the last measurement of 240 min (n = 8 for vehicle; n = 11 for AMG 517; *P < 0.05, two-way ANOVA with Bonferroni post hoc test; error bars = SD). (B) In a setup similar to that used in (A), although ketamine (50 mg/kg i.p.) induced hypothermia in TRPV1 knockout (Trpv1^–/–) mice, AMG 517 (1 mg/kg i.p.) did not prevent ketamine-induced hypothermia at all of the time points tested (n = 7, two-way ANOVA showed no significant difference between the groups; error bars = SD).