Fig. 1.
Oxygen–glucose deprivation (OGD) and cell viability. (A) All OGD groups of Neuro2A cells stably transfected with mouse κ-opioid receptor–tdTomato fusion protein (N2A-mKOR-tdT) cells maintained with 95% N2 and 5% CO2 from 0.5 to 12 h had significantly decreased partial pressure of oxygen (mmHg) in culture medium compared with that in the control group without OGD (n = 6 per group). (B) There was no significant difference among partial pressure of carbon dioxide (mmHg) in culture medium with OGD of N2A-mKOR-tdT cells from 0.5 to 12 h (n = 6 per group). OGD-induced time-dependent decrease of cell viability in both (C) N2A-mKOR-tdT and (D) Neuro2A cells stably transfected with Flag-tagged mouse κ-opioid receptor (N2A-mKOR-FLAG) cells after 4 h of treatment (n = 12 per group). Data are shown as mean ± SD. *P < 0.001.