Fig. 4.
Effects of diabetes and isoflurane treatment on the levels of reduced form of nicotinamide adenine dinucleotide during ischemia and reperfusion. All hearts were stabilized in Langendorff apparatus for 30 min (baseline) and perfused with the buffer with or without isoflurane before 30 min of global ischemia (ischemia) followed by 2 h of reperfusion (reperfusion). Control = C57BL/6 mouse hearts undergoing ischemia/reperfusion injury: db/db = C57BL/6 mouse hearts undergoing ischemia/reperfusion injury; isoflurane = C57BL/6 mouse hearts treated with 1.4% isoflurane before ischemia; db/db+isoflurane = db/db mouse hearts treated with 1.4% isoflurane before ischemia. *P < 0.05 versus control groups; †P < 0.05 versus db/db groups; #P < 0.05 versus isoflurane groups (n = 9 mice in control, 8 mice in db/db group, 9 mice in isoflurane, and 8 mice in db/db+isoflurane group).