Fig. 4.
Effects of bupivacaine (Bupi) on 3´,5´-cyclic adenosine monophosphate (cAMP) concentration in isolated mouse cardiomyocytes. Original registrations of time course of cAMP concentrations normalized to predrug control values in Förster Resonance Energy Transfer measurements. (A) Response to 100 nM isoprenaline (Iso), (B) response to 100 nM isoprenaline after pretreatment with 5 µM bupivacaine, and (C) response to 5 µM bupivacaine. Cells were exposed at the end of the experiment to 100 µM 3-isobutyl-1-methylanxthine plus 10 µM forskolin (IBMX + FSK). (D) Overview of mean values ± SD for cAMP in cells exposed to 100 nM isoprenaline and subsequently to 100 µM IBMX + FSK under control conditions (open bars) or in the presence of 5 µM bupivacaine (closed bars). ***P < 0.001 versus pre-Iso, two-way ANOVA with Bonferroni posttest. CFP = cyan fluorescent protein; YFP = yellow fluorescent protein.

Effects of bupivacaine (Bupi) on 3´,5´-cyclic adenosine monophosphate (cAMP) concentration in isolated mouse cardiomyocytes. Original registrations of time course of cAMP concentrations normalized to predrug control values in Förster Resonance Energy Transfer measurements. (A) Response to 100 nM isoprenaline (Iso), (B) response to 100 nM isoprenaline after pretreatment with 5 µM bupivacaine, and (C) response to 5 µM bupivacaine. Cells were exposed at the end of the experiment to 100 µM 3-isobutyl-1-methylanxthine plus 10 µM forskolin (IBMX + FSK). (D) Overview of mean values ± SD for cAMP in cells exposed to 100 nM isoprenaline and subsequently to 100 µM IBMX + FSK under control conditions (open bars) or in the presence of 5 µM bupivacaine (closed bars). ***P < 0.001 versus pre-Iso, two-way ANOVA with Bonferroni posttest. CFP = cyan fluorescent protein; YFP = yellow fluorescent protein.

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