Figure 2. Schematic diagram of the experimental protocol used to study the role of isoflurane and KATPchannels in stunned myocardium produced by multiple left anterior descending (LAD) coronary artery occlusions and reperfusion. All dogs were subjected to five 5-min periods of LAD occlusion (OCC), interspersed with 5-min periods of reperfusion and followed by a final 180 min of reperfusion. Percentage segment shortening (%SS), systemic and coronary hemodynamics, arterial blood gases, blood glucose, and regional myocardial blood flow were measured at the indicated time intervals.