Figure 6. Bisindolylmaleimide blocks tetradecanoyl phorbol acetate (TPA)-associated reductions in mu opioid receptor (MuOR) mRNA. SH-SY5Y cells were pretreated with bisindolylmaleimide (1 micro Meter final concentration, BIS) for 20 min before the administration of TPA (100 nM) or ethanol vehicle control. Cells were harvested after 4 h. (A) Representative autoradiograms from one experiment. The experimental treatments are indicated. (B) Graph representing the levels of MuOR mRNA in SH-SY5Y cells after experimental treatment as indicated. Data from three independent experiments (n = 15–22 per data point) were normalized to the control value, pooled, and plotted as the mean +/- SE. **Indicates different from control, at P <or= to 0.01, with other values not significantly different from control as determined by analysis of variance with Dunn's test for multiple comparisons.