Photomotor responses in 7 days postfertilization zebrafish larvae. Activity integration (blue lines) is a Zebralab experimental output totaling the number and intensity of pixel changes in a designated image area between sequential infrared video sweeps during a 0.2-s experimental epoch (each epoch includes five video sweeps lasting 0.04 s). (A) Average activity integration for eight larvae in E3 buffer with 0.2% DMSO (control conditions) is plotted at 0.2-s intervals during a single photomotor response trial. A 500-lux white light stimulus was activated at 5 s and discontinued at 5.2 s (pink bar). Note that activity dramatically increased during the photic stimulus and diminished within 1 s. (B) Activity of a single larva during a series of 10 photomotor response trials, with 3-min intervals between trials, is shown. Pink bars indicate photic stimuli. The purple dashed line indicates the upper 95% CI for mean baseline activity during all 10 prestimulus epochs (5 s each). Activity during and after photic stimuli was consistently above the 95% baseline threshold in all trials, while varying in magnitude from trial to trial.