The relationship between temporal summation of pain (TSP) and pain relief is mediated by default mode network–descending antinociceptive pathway dynamic functional connectivity (DMN-Des dFC). These variables were entered as predictors into a model with pain relief as the outcome variable. The direct path and indirect effect are shown. *P < 0.05. mPFC, medial prefrontal cortex; PAG, periaqueductal grey; PCC, posterior cingulate cortex; RVM, rostral ventral medulla.