Figure 3. Effect of P450 inhibitors on anesthetic defluorination by microsomes from human kidneys. A panel of inhibitors, each selective for a particular isoform of human P450, was used at optimal concentrations. The inhibitor and the isoform that it inhibits are shown on the left of the graph. These included coumarin (P450 2A6), orphrenadrine (P450 2B6), sulfaphenazole (P450 2C9/10), quinidine (P450 2D6), diethyldithiocarbamate (P450 2E1), troleandomycin (P450 3A), midazolam (P450 3A), and lauric acid (P450 4A). Shown are the results of triplicate determinations for methoxyflurane (solid bars) and sevoflurane (open bars).