Figure 2. Measurements of halothane were sampled every minute for 5 min and then every 5 min thereafter to determine the time course of equilibration of the volatile anesthetic in the superfusing artificial cerebrospinal fluid in the recording chamber. Shown is an example of halothane concentrations when setting vaporizer dial concentration from 0% to 2%(n = 5). Stabilization of halothane occurred after 25 to 30 min. Millimolar concentrations were calculated to equivalent partial pressures.