Figure 1. Average depressant effects on peak Na sup + current by equianesthetic concentrations of 1.2 mM halothane (A) or 1 mM isoflurane (B). Sample peak Na sup + current traces (leak subtracted) are shown for control and after exposure to halothane and isoflurane. Calibration bars denote 500 pA and 5 ms. Data were recorded during a 50-ms test pulse to -20 mV from a V^Hof -80 mV. Anesthetic effects are shown for untreated cells, in the presence of intracellular guanosine 5'-O-(2-thiodiphosphate)(GDP beta S), after pretreatment of cells with pertussis toxin (PTX), in combination with GDP beta S and PTX, and in the presence of bisindolylmaleimide (BIS). Averaged data of seven to nine cells per experimental group are shown. Error bars denote SEM;* significantly different than control;(dagger) significantly different than halothane;# significantly different than isoflurane.