Figure 3. Increase in fluorescence after mixing Dns-C6-Cho with membranes containing the nAcChoR. Final concentrations were 2.0 micro Meter Dns-C6-Cho and 0.4 micro Meter agonist binding sites. The same experiment is displayed in A and B but on different time scales. The component reflecting the binding of Dns-C6-Cho to receptors in the high-affinity slow desensitized state (approximately 20% of all receptors) occurs over 10–15 ms and is indicated by the arrow in A. The slower time scale in B demonstrates conversion of the low-affinity resting-state receptors to the high-affinity slow desensitized state (agonist-induced desensitization).