Figure 8. Change in the relative rates (rate/rate^control) of the second, third, and fourth components after equilibration of receptor membranes with clinically relevant concentrations of isoflurane (A) or butanol (B). The dashed lines indicate the EC⁵⁰s for anesthesia in tadpoles, which are 0.3 mM and 12 mM for isoflurane and butanol, respectively. Final concentrations were 2.0 micro Meter Dns-C⁶-Cho and 0.4 micro Meter agonist binding sites. Control rates (no anesthetic) are given in Table 1.