Figure 2. Plasma membrane Calcium²+-ATPase pumping activity in cerebral SPM vesicles from rats in three treatment groups: control (C), anesthetized (A), and recovered (R). Calcium²+ transport rates (nmoles *symbol* mg protein sup -1 *symbol* min¹) were derived from time-course data displayed in Figure 1. Plasma membrane Calcium²+-ATPase pumping (open columns) was significantly reduced to 71% of control (P < 0.01) in rats killed while anesthetized, compared with C and R treatments. Orthovanadate (hatched columns) inhibited Calcium²+ uptake by SPM vesicles in all treatment groups. Error bars indicate 95% confidence limits derived from ANOVA.