Figure 2. Binding of¹²⁵Iodine-BHSP to chick brain membranes in the presence (closed circles) and absence (open circles) of 1 mM bupivacaine as saturation isotherms (A) and Scatchard plots (B). Results from two experiments, each done in duplicate. Hill plots of the data presented in A show slopes not significantly different from unity (n^H= 1.08 and 0.97 in the absence and presence of bupivacaine, respectively). Dashed lines in B indicate 95% confidence intervals.