![Figure 4. Time course of the change in [Ca2+]inmeasured with fura-2 in P388D1cells. Substance P (10 sup -8 M) was perfused over the cells during the time of the heavy horizontal bar. This typical result from a single run over an optical field containing 12–18 cells shows the peak and plateau phases of increased [Ca2+]inin the absence and presence of 2 mM bupivacaine. Experiments were conducted at room temperature (approximately 24 degrees Celsius).](https://asa2.silverchair-cdn.com/asa2/content_public/journal/anesthesiology/82/1/10.1097_00000542-199501000-00021/2/21ff4.png?Expires=1716640714&Signature=gNmKFvuT29mU3pQeLjPeIB~UxBOxF~uCv1DqFqp9c~30dcyNuMY2YvIYSlUZttKPsgs54q~ew-tM7kLIn1WKbwg2a3rM3cCpnTk3CwSKjH4PMpKgMCYgrNg3kxqShcG7Ae36QKkBHOZ7UBRVxFST-ZlyqKGpjpqt4FUT9YGWYkXqlvl8byF-bBaanU0lhPaUqRp12J4aLYBooX7okL-unEQH3V6oJgqEpyhJ9X-BT4sWn53aDdAaFHCBUbAGMGlfBrsD1VgF6ITms0asoqz3h4fQq7T90~hAK7xTWNgW8h7oQ9qzUW2W16cKKd3IwcMwVfRBLFpZIyrVJqhYxPWAnA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 4. Time course of the change in [Ca2+]inmeasured with fura-2 in P388D1cells. Substance P (10 sup -8 M) was perfused over the cells during the time of the heavy horizontal bar. This typical result from a single run over an optical field containing 12–18 cells shows the peak and plateau phases of increased [Ca2+]inin the absence and presence of 2 mM bupivacaine. Experiments were conducted at room temperature (approximately 24 degrees Celsius).
