Figure 5. Effects of halothane (H) on the capacitative Calcium2+influx in BAE cells after cell depolarization. (A) Effect of 2 mM halothane on depolarized cells after pool depletion via 10nM bradykinin receptor stimulation. The cells were depolarized using Potassium+-Earle's solutions. Perfusion protocol as in Figure 3A. In contrast to the results presented in Figure 3A, the removal of halothane failed to initiate an additional Calcium2+increase under Potassium+-Earle's conditions. The resulting Calcium2+increase observed when cells were repolarized by perfusing with a standard Earle's solution (E) confirmed the voltage dependence of the capacitative Calcium2+influx in these cells. (B) Effect of 2 mM halothane on depolarized cells treated with 500 nM of the Calcium2+pump inhibitor thapsigargin. Bath application of halothane was first carried out under standard Earle's conditions (E). Halothane was then applied on depolarized cells bathed in a Potassium+-Earle's external solution. Halothane was ineffective in reducing the Calcium2+level under these conditions.