Figure 7. Correlation between Potassium(Calcium2+) channel activity and cytosolic Calcium2+fluctuations in bradykinin (BK)-stimulated cells exposed to pulse applications of halothane (H). Combined cell-attached + fura-2 experiment carried out on BAE cells stimulated with 10 nM bradykinin. The patch pipette was filled with a 200 mM KCl + 1 micro Meter free Calcium2+solution, and the bath contained a normal Earle's medium. The potential in the patch electrode was 30 mV throughout. Potassium(Calcium2+) channel activity was abolished systematically in the presence of halothane, despite minor variations of the Calcium2+level during the first perfusion with halothane. In subsequent halothane pulses, halothane caused a decrease in internal Calcium2+concentration that was correlated with a marked reduction in the Potassium(Calcium2+) channel activity.