Figure 1. Activated clotting time (ACT) vs. heparinase I (Neutralase) concentration determined after incubation at 37 degrees C for 0 (activated clotting time measured immediately after heparinase I treatment), 5, 10, 15, or 20 min. Results are presented as mean+/- SD, and n = 4/group. *P < 0.05, **P < 0.01 compared to activated clotting time values corresponding to the higher concentrations of heparinase I in this same curve;#P < 0.05, compared to equiconcentration values with 5–20 min of incubation.