Figure 2. Dose-dependent effect of halothane on the uptake of glutamate and gamma aminobutyeric acid (GABA) by cultured hippocampal astrocytes. Halothane (0–4%) was applied using the gas-flow method. The amounts of [sup 14 C]glutamate (closed circles) and [sup 3 H]GABA (open circles) taken up by the cells for 5 min were measured simultaneously with the double-labeled method (mean +/- SD; n = 4). *P < 0.05, **P < 0.01 with the value of control, 0% halothane, by one-way analysis of variance followed by Fisher's paired least-squares test.