Figure 7. Summary of effects of halothane on Ca²⁺clearance (A) and on baseline and peak relative [Ca²⁺]ⁱ(B) in wild-type (n = 6), antisense PMCA2 oligodeoxyribonucleotide-treated (n = 8), and scrambled base-treated (n = 6) neurons. Each cell was stimulated with N-methyl-D-aspartate after sequential superfusion for 10 min or more with buffer (c1), buffer with 0.15 mM halothane (h1), buffer (c2), buffer with 0.30 mM halothane (h2), and buffer (c3). Halothane at concentrations equivalent to 0.5 and 1 minimum alveolar concentration prolonged Ca²⁺clearance and elevated baseline and peak [Ca²⁺]ⁱin antisense-treated neurons. These same Ca²⁺parameters were affected in wild-type and scrambled base-treated neurons by the higher but not the low halothane concentration. *Values different from all other groups within each cell type. dagger A value different from all other groups within the antisense-treated cells (P < 0.01).