Figure 9. Effects of halothane on Ca²⁺clearance (A) and on baseline and peak relative [Ca²⁺]ⁱ(B) in wild-type (PC6, n = 6), antisense PMCA1-transfected (RSV-9, n = 8) pheochromocytoma cells and sense PMCA1-transfected (RSV-14, n = 6) pheochromocytoma cells. Each cell was stimulated with bradykinin (10^-3M applied for 3 s) after sequential superfusion for 20 min with buffer (c1), buffer with 0.03 mM halothane (h1), buffer (c2), buffer with 0.30 mM halothane (h2), and buffer (c3). Halothane at concentrations equivalent to 0.1 MAC prolonged Ca²⁺clearance and elevated baseline and peak [Ca²⁺]ⁱin antisense-transfected cells with blocked production of PMCA1, but not in wild-type and sense-transfected cells. These same Ca²⁺parameters were altered in all three cell types by 0.30 mM halothane. *Values different from all other groups within each cell type. [dagger]A value different from all other groups within the antisense-transfected cells (P < 0.01).