Figure 7. Fraction of BSA (22 [micro sign]M) molecules unfolded (fu) as a function of temperature in 130 mM NaCl, 20 mM sodium phosphate, pH 7.0, followed by the disappearance of the ellipticity at 270 nm. Halothane (filled circles) and isoflurane (filled squares) shift the denaturation transition to higher temperatures in a concentration-dependent manner. Solid curves represent fits to the data, determined as described in methods.