Figure 1. Current-voltage relations of cloned tandem pore domain K⁺channels. Raw signals without leakage subtraction are shown. Voltage pulses were from -140-+40 mV in 20-mV increments from a holding potential of -80 mV. (A-E) Current-voltage relations of TASK, TREK-1, TOK1, ORK1, and TWIK-1 cRNA-injected oocytes in the absence and presence of 1 mM bupivacaine are shown. (F) Current-voltage curves from control saline-injected oocytes in the presence and absence of 1 mM bupivacaine are also shown. Data are mean +/− SD.