Figure 4. Depolarization of TASK cRNA-injected and saline-injected oocytes during exposure to local anesthetics. (A) Representative tracings of the resting membrane potential before, during, and after treatment with 1 mM tetracaine or 1 mM bupivacaine. Local anesthetics were applied for 30 s (indicated by vertical bars). Resting membrane potentials of TASK cRNA-injected oocytes and saline-injected control oocytes were -69 +/− 12 mV (n = 21) and -35 +/− 12 mV (n = 21), respectively (P < 0.05). (B) Depolarization in millivolts of both TASK cRNA-injected and control oocytes. Data are expressed as the mean +/− SD. The numbers of parentheses above the error bars refer to the number of oocytes studied. *P < 0.05 treatment with local anesthetic of TASK cRNA-injected oocytes versus control oocytes.