Figure 1. A representative recording of the effect of 3.5 mM sevoflurane on a muscle fiber bundle shows a calcium-induced force transient (A), which is normalized to maximal force obtained in the presence of 24.9 [micro sign]M Ca (2+) and indicated by the value 1.0. The [Ca2+]-force relation is shown in B. The Ca2+transient is derived from the force transient based on the relation defined between [Ca2+] and force of the same fiber (C). As a control for the force transient, the caffeine (30 mM) transient of the same fiber is shown. The control for the [Ca2+]-force relation without caffeine or a volatile anesthetic is shown in B.