Fig. 9. The effects of (  A ) tetrodotoxin (TTX), veratridine (VTD), and (  B ) ouabain (Oua) on carbachol-induced activation of extracellular signal-regulated kinases (ERKs) in PC12 cells. The cells were treated in the presence or absence of 1 × 10−6M tetrodotoxin, 1 × 10−6M veratridine, or 5 ×10−3M ouabain for 10 min and then stimulated with or without 1 mM carbachol for 2 min. Extracted proteins were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and analyzed by immunoblotting with anti-ERK antibody. The upper two arrows on the left indicate ERK1 (  , active forms of ERK1) and the lower two arrows indicate ERK2 (  , active forms of ERK2). 
Fig. 9. The effects of (  A ) tetrodotoxin (TTX), veratridine (VTD), and (  B ) ouabain (Oua) on carbachol-induced activation of extracellular signal-regulated kinases (ERKs) in PC12 cells. The cells were treated in the presence or absence of 1 × 10−6M tetrodotoxin, 1 × 10−6M veratridine, or 5 ×10−3M ouabain for 10 min and then stimulated with or without 1 mM carbachol for 2 min. Extracted proteins were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and analyzed by immunoblotting with anti-ERK antibody. The upper two arrows on the left indicate ERK1 ( 
formula
, active forms of ERK1) and the lower two arrows indicate ERK2 ( 
formula
, active forms of ERK2). 
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