Fig. 5. Specificity of AP-1 binding to the tissue factor AP-1 (distal [D]) regulatory element in rat lung nuclear extract isolated from sham-operated control (C) rats and rats 2 h after Noble-Collip drum trauma (T). Binding reactions were carried out with AP-1 (D) probe containing native distal (D) and mutated (inactive) proximal AP-1 sites. Nuclear extracts were incubated during the following conditions from left to right: Control lane with no nuclear extract (−NE); labeled AP-1 (D) probe, which showed an increase in its binding post-trauma compared with sham-operated controls. Lanes 4 and 5 show complete inhibition by competitor (unlabeled AP-1 probe) in 100-fold excess plus labeled AP-1 probe). Lanes 6 and 7 show labeled AP-1 (D) probe with nuclear extract from other control and trauma rats.