Fig. 6. The effects of dibucaine, tetracaine, EGTA, nifedipine, and mitogen-activated/extracellular receptor–regulated kinase (MEK) inhibitor on the KCl-induced expression of c-Fos. The cells were incubated with 50 mM KCl for 60 min at 37°C after pretreatment with 20 μM dibucaine, 200 μM tetracaine, 1 mM EGTA, or 5 μM nifedipine for 10 min, and with 50 μM MEK inhibitor (PD 98059) for 30 min. The expression of c-Fos was detected as described in Materials and Methods. (  A ) Protein blots were probed with anti-c-Fos antibody. The typical results of three independent experiments are shown. (  B ) A densitmetric analysis of the expression of c-Fos. c-Fos expression is expressed as a percentage of that caused by 50 mM KCl without pretreatment (positive control). Values are mean ± SEM of three independent experiments. *Different from positive control at  P < 0.05. 

Fig. 6. The effects of dibucaine, tetracaine, EGTA, nifedipine, and mitogen-activated/extracellular receptor–regulated kinase (MEK) inhibitor on the KCl-induced expression of c-Fos. The cells were incubated with 50 mM KCl for 60 min at 37°C after pretreatment with 20 μM dibucaine, 200 μM tetracaine, 1 mM EGTA, or 5 μM nifedipine for 10 min, and with 50 μM MEK inhibitor (PD 98059) for 30 min. The expression of c-Fos was detected as described in Materials and Methods. (  A ) Protein blots were probed with anti-c-Fos antibody. The typical results of three independent experiments are shown. (  B ) A densitmetric analysis of the expression of c-Fos. c-Fos expression is expressed as a percentage of that caused by 50 mM KCl without pretreatment (positive control). Values are mean ± SEM of three independent experiments. *Different from positive control at  P < 0.05. 

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