Figure 7. The effects of propofol on voltage-gated Ca²⁺currents. (A) Representative traces of Ca²⁺currents elicited by voltage steps to -20 mV from the holding potential (Vh) of -80 mV before (control), during, and after (washout) application of 10^-4M propofol. Propofol reversibly inhibited voltage-gated Ca²⁺currents. (B) Representative time course of propofol-induced inhibition of Ca²⁺currents. Voltage-step commands to -20 mV from -80 mV were applied every 10 s, and the peak Ca²⁺currents are plotted against time. (C) The current-voltage relation of Ca²⁺currents recorded before and during application of 10^-4M propofol. (D) The dose-response relation of the propofol-induced inhibition of the Ca²⁺current. The data are shown as a percentage of the total currents measured just before propofol application (the mean +/- SD of 10 experiments). **Significant (P < 0.01) by the Wilcoxon signed-rank test.