Fig. 4. Enflurane had a significant depressant effect on synaptic currents in motor neurons evoked by dorsal horn stimulation under voltage clamp at a holding potential of −60 mV. (A ) Sample records from a motor neuron in a slice from a 1-day-old mouse. Enflurane at one minimum alveolar anesthetic concentration for 10 min reversibly decreased both amplitude and area of the inward currents evoked by stimulation in the dorsal horn. The effect was fully reversible on washing for 20 min. (B ) Histogram summarizing the depressant effect of enflurane on excitatory postsynaptic currents in six cells tested. The synaptic responses were depressed to about half of their size at an enflurane concentration of one minimum alveolar anesthetic concentration. Data are means of six cells, each from a different slice; error bars are SEMs.