Fig. 4. Effects of acetylcholine application times during perfusion of dissociative anesthetics on currents of human neuronal nicotinic acetylcholine receptors. The current responses of the α²β⁴, α³β⁴, and α⁴β²channels were evoked by repeated applications of acetylcholine during continuous perfusion of 10 μM dizocilpine, 10 μM ketamine, and 100 μM ketamine, respectively (n = 4–5).