Fig. 1. Effects of 46 mM K+(solid bar) on glutamate release from perfused rat cerebrocortical slices. Slices were challenged twice (S1and S2); challenges were separated by 30 min. Release is expressed relative to the mean of the first three fractions collected during S1or S2. The effects of anesthetic agents and bicuculline added for various times after S1on S2were investigated. S2/S1ratios are calculated from the area under both stimulation profiles. Data are mean ± SEM and are from the 96 control experiments performed during the entire study.
