Fig. 7. Immunoblot analysis of Gsαin ventricular myocardium from control, 3-h septic, and 6-h septic rabbits. Two different antisera, (A ) 3A-160 and (B ) RM/1, were used to identify Gsα. (Upper traces ) Representative Western blot indicating marked decreases in expression of 45-kDa band in sepsis. (Lower traces ) Summary comparing the immunostained band for Gsαin the three groups of ventricular myocardium. Densitometric results are expressed as percent of the band obtained with control in each experiment. Mean of data from four experiments are presented, with SD shown by vertical lines. ***Significant difference from control (P < 0.001).