Fig. 6. Effects of isoflurane on uptake–internalization of extracellular calcium and iron in cells exposed to iron. Radioactive cations (59Fe or 45Ca) were added to the culture medium immediately before exposure to 300 μm FeSO4–FeCl3. The amount of cation uptake was assessed after 30-min incubation at 37°C. (A ) Neither iron exposure alone (Fe) or in the presence of 1.2 mm isoflurane (Fe + Iso) affected the rate of calcium uptake as compared with controls receiving no treatments (Control). (B ) Iron exposure caused an increase in uptake–internalization of extracellular iron. The increase was less in cultures treated with isoflurane during iron exposure. Values are mean ± SD (n = 14 culture wells for 45Ca uptake study, n = 6 for 59Fe uptake study). aP less than 0.05 versus untreated controls; bP less than 0.05 versus 59Fe alone. CPM = counts/min.