![Fig. 2. μ-Opioid receptor (MOR) activation induces Elk-1-mediated transcription. Cells were pretreated with pertussis toxin (PTX; 100 ng/ml; 24 h) or PD98059 (50 μm; 2 h), and then stimulated with 1 μm [D-Ala2, N -Me-Phe4,Gly-ol5]enkephalin (DAMGO) or 1 μm morphine. The ratio of normalized luciferase activity to that of nonstimulated control cells is expressed as mean ± SD from three separate experiments done in duplicate (*P < 0.05 vs. nonstimulated control; #P < 0.05 vs. cells stimulated by the same agonist without pretreatment).](https://asa2.silverchair-cdn.com/asa2/content_public/journal/anesthesiology/95/4/10.1097_00000542-200110000-00030/2/30ff2.png?Expires=1716608962&Signature=O3k9tp-5ZgJ~zn3jCZdPLjcf~5g0QaMbiRn7ylADbj9k~ZMqMS2EIEjvmhGwUmFI3jdAZBgpAn2FkLuPVMrTOvscOtawQDbxGSViU3xgOAXks5hGNqf1iM~etABmDroLM7rhr-qzxlhj4wTxUL11oVdfTaoOOTCB~ccWPwyQ5UxmXmOuXCkOvEYKkQzQsmemFCn8O0e5~AR7yLXz7NBCmMDn2LqZD0RdhtGm0yefzBwYv0BdwlQL46SVWQuLdNOyZM2jcS1HlSwM8gSs4xfsvtOV~Eoo4ui0KXd31Ygm-ULyvXzIoW5FRSGRN4a5r8H0j770KuLig4SVtkk1SbOm6g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fig. 2. μ-Opioid receptor (MOR) activation induces Elk-1-mediated transcription. Cells were pretreated with pertussis toxin (PTX; 100 ng/ml; 24 h) or PD98059 (50 μm; 2 h), and then stimulated with 1 μm [D-Ala2, N -Me-Phe4,Gly-ol5]enkephalin (DAMGO) or 1 μm morphine. The ratio of normalized luciferase activity to that of nonstimulated control cells is expressed as mean ± SD from three separate experiments done in duplicate (*P < 0.05 vs. nonstimulated control; #P < 0.05 vs. cells stimulated by the same agonist without pretreatment).
