Fig. 9. (A ) ERK phosphorylation induced by several concentrations of tetracaine. PC12 cells were exposed to 0.2, 0.5, or 1 mm tetracaine for indicated times, and then ERK phosphorylation was analyzed using phospho-ERK antibody. (B  and C ) The effect of PD98059 on lower concentration of tetracaine-induced lactate dehydrogenase (LDH) release. PC12 cells were pretreated with (PD98059) or without (control) 50 μm PD98059 for 60 min and then exposed to 0.2 or 0.5 mm tetracaine for indicated times, respectively. LDH activity released in the media was analyzed. Data are mean ± SD from three different experiments, each performed in triplicate. *P  <  0.05 versus  control at the same time points. (D ) The effect of PD98059 on 0.5 mm tetracaine-induced JNK phosphorylation. PC12 cells pretreated with PD98059 were exposed to 0.5 mm tetracaine, and Western blot analysis was performed with phospho–stress-activated protein kinases–JNK antibody.

Fig. 9. (A ) ERK phosphorylation induced by several concentrations of tetracaine. PC12 cells were exposed to 0.2, 0.5, or 1 mm tetracaine for indicated times, and then ERK phosphorylation was analyzed using phospho-ERK antibody. (B  and C ) The effect of PD98059 on lower concentration of tetracaine-induced lactate dehydrogenase (LDH) release. PC12 cells were pretreated with (PD98059) or without (control) 50 μm PD98059 for 60 min and then exposed to 0.2 or 0.5 mm tetracaine for indicated times, respectively. LDH activity released in the media was analyzed. Data are mean ± SD from three different experiments, each performed in triplicate. *P  <  0.05 versus  control at the same time points. (D ) The effect of PD98059 on 0.5 mm tetracaine-induced JNK phosphorylation. PC12 cells pretreated with PD98059 were exposed to 0.5 mm tetracaine, and Western blot analysis was performed with phospho–stress-activated protein kinases–JNK antibody.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal