Fig. 2. Flow cytometric analysis of fluorescein isothiocyanate (FITC)–triflavin binding to human platelets in the absence or presence of various concentrations of midazolam (15 and 26 μm). (A ) The solid line represents the fluorescence profiles of FITC–triflavin (2 μg/ml) in the absence of midazolam as a positive control; (B ) in the presence of EDTA (5 mm) as the negative control; or in the presence of (C ) 15 μm and (D ) 26 μm midazolam. The profiles are representative examples of five similar experiments.