Fig. 6. The effect of methohexital on nuclear factor κB (NF-κB) DNA binding after tumor necrosis factor α (TNF-α) stimulation. Jurkat cells were treated for 1 h with methohexital at concentrations indicated (lanes 3–6) and subsequently stimulated with 5 U/ml of TNF-α for 1 h (lanes 2, 5, and 6) or the respective volumes of ppH2O as vehicle control. Filled arrowhead = position of NF-κB DNA complexes; open circle = a nonspecific activity binding to the probe; open arrowhead = unbound oligonucleotide. The results are representative of six independent experiments.