Fig. 9. The effect of thiopental on tumor necrosis factor α (TNF-α)-induced interleukin-8 production in primary CD3+T lymphocytes, isolated from the blood of healthy donors and pretreated with different concentrations of thiopental (100, 400, or 1,000 μg/ml) 1 h before stimulation with TNF-α (100 U/ml) for 24 h. The cell culture supernatant was collected and analyzed for the concentration of interleukin-8 by enzyme-linked immunosorbent assay. *P < 0.05 compared with TNF-α stimulation alone. Data represent the median, 25–75%, and 95% confidence interval of six to eight experiments in each group.