Fig. 2. Effect of isoflurane on activation of adenosine triphosphate–sensitive potassium current (I^KATP) by pinacidil. (A ) Traces of I^KATPactivated by pinacidil after pretreatment with isoflurane. The voltage protocol was as described in figure 1. Isoflurane alone did not activate I^KATP. After a 10-min pretreatment with isoflurane, activation of I^KATPby 5 μm pinacidil was monitored in the continued presence of 0.5 mm isoflurane. Current activated by pinacidil was blocked by 0.5 μm glibenclamide. (B ) Corresponding time course of I^KATPactivation by pinacidil after pretreatment and in the presence of isoflurane. The 20-min control dialysis of the cell with the pipette solution containing 0.5 mm ATP was allowed before isoflurane, and subsequently isoflurane and pinacidil were added to the bath solution. Current activated by pinacidil was blocked by glibenclamide (Glib).