Fig. 4. Stimulation of receptor associated protein tyrosine kinases (PTKs) with epidermal growth factor (EGF), nerve growth factor (NGF), or insulin attenuates the effects of genistein and isoflurane on whole cell KATPcurrent (IKATP). (A ) Time course of IKATPin the presence of EGF (10 ng/ml) shows a marked attenuation of current activation by 50 μm genistein and potentiation by 0.5 mm isoflurane (compare with fig. 3A). (B ) Summary of the effects of EGF (10 ng/ml), NGF (0.5 μg/ml), and insulin (5 μm) on IKATPactivation by genistein, and potentiation by isoflurane (compare with fig. 3B). Insulin did not prevent isoflurane po-tentiation. *P < 0.05 versus genistein, n = 3 cells in each group. (C ) Time course of IKATPactivation by genistein and isoflurane. Activated current was suppressed by 20 ng/ml EGF applied to the bath solution at the current plateau. [ATP]in= intracellular adenosine triphosphate concentration.