Fig. 7. Effect of isoflurane on the KATPchannel activated bimakalim and 2,4-dinitrophenol (DNP). Channel activity was monitored in the inside-out patch mode. (A ) Summary of the effect of isoflurane (0.5 mm) on the KATPchannel activated by 5 μm bimakalim (n = 7). Control condition was obtained in 3.0 mm adenosine triphosphate (ATP). (B ) Summary of the effect of isoflurane (0.5 mm) on the KATPchannel activated by DNP (150–200 μm) (n = 8). Control condition was obtained with 0.8–1.0 mm ATP in the internal solution. DNP was added in the internal side of the membrane. *P < 0.05 versus control.