Fig. 8. Opening of the KATPchannel by isoflurane during protein kinase C (PKC) activation. KATPcurrent was monitored in the whole cell mode. Current amplitude was measured at the end of the 100-ms test pulse to 0 mV from a −40-mV holding potential. The intracellular adenosine triphosphate concentration was set at 0.5 mm. (A ) Isoflurane (1.0 mm) alone was unable to elicit KATPcurrent activation. (B ) During continuous PKC activation by 12,13-dibutyrate (PDBu; 1 μm), isoflurane activated KATPcurrent, which was subsequently blocked by glibenclamide (500 nm).